重组人RPA2蛋白

产品详情

  • 产品名称
    重组人RPA2蛋白
  • 蛋白种属
  • 表达宿主
    大肠杆菌
  • Unitprot ID
  • 克隆构建
    80-270 aa encoded by NM_002946 was inserted into PET28a vector
  • 蛋白活性
    Not tested.
  • 蛋白别名
    REPA2; RPA32; RPA34
  • 蛋白全名
    Replication protein A 32 kDa subunit
  • 蛋白描述
    As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {PubMed:15205463, PubMed:17765923, PubMed:17959650, PubMed:19116208, PubMed:20154705, PubMed:21504906, PubMed:2406247, PubMed:24332808, PubMed:7697716, PubMed:7700386, PubMed:8702565, PubMed:9430682, PubMed:9765279}.
  • 发货形式
    Protein lyophilized in sterile PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 300 mM Imidazole, pH 8.0). Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization.
  • 内毒素
    Please contact the lab for more information.
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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