重组人SPHK1蛋白

产品详情

  • 产品名称
    重组人SPHK1蛋白
  • 蛋白种属
  • 表达宿主
    杆状病毒 - 昆虫细胞
  • Unitprot ID
  • 蛋白序列
    Met 1 - Leu 384
  • 克隆构建
    A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
  • 蛋白纯度
    > 85 % as determined by SDS-PAGE
  • 蛋白活性
    The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
  • 分子量
    The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
  • 蛋白别名
    SK 1; RP23-193A16.4; SPK1; 1110006G24RIK; SPHINGOSINE KINASE 1; SPHK1; SK1; SPHK; SPK 1
  • 蛋白描述
    SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
  • 发货形式
    Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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