重组人SPHK1蛋白
- 蛋白种属:人
- 表达宿主:杆状病毒 - 昆虫细胞
- Unitprot ID:Q9NYA1
产品详情
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产品名称
重组人SPHK1蛋白
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蛋白种属
人
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表达宿主
杆状病毒 - 昆虫细胞
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Unitprot ID
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蛋白序列
Met 1 - Leu 384
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克隆构建
A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
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蛋白纯度
> 85 % as determined by SDS-PAGE
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蛋白活性
The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate.
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分子量
The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
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蛋白别名
SK 1; RP23-193A16.4; SPK1; 1110006G24RIK; SPHINGOSINE KINASE 1; SPHK1; SK1; SPHK; SPK 1
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蛋白描述
SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
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发货形式
Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!