- 抗体应用:ELISA, IF, WB
- 特异性:Human,Mouse,Rat,Zebrafish; other species not tested.
LC3 Rabbit Polyclonal antibody. Positive IF detected in Starvation treated HEK-293 cells. Positive WB detected in Chloroquine treated HeLa cells, Chloroquine treated HEK-293 cells, human brain tissue, mouse brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 16 kDa
ELISA, IF, WB
Human,Mouse,Rat,Zebrafish; other species not tested.
LC3, LC3A, LC3A/B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A
This antibody was obtained by immunization of LC3 recombinant protein (Accession Number: XM_047440559). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.