MLH1 antibody

多克隆

兔

兔IgG

MLH1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, human testis tissue, Jurkat cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 85 kDa

ELISA, WB, IHC, IP

Human,Mouse,Rat; other species not tested.

COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1, MutL protein homolog 1

This antibody was obtained by immunization of MLH1 recombinant protein (Accession Number: NM_000249). Purification method: Antigen affinity purified.

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

MLH1, also named as CocA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. This antibody is specific to MLH1.

• Jin P, Lu XJ, Sheng JQ. Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells. Cancer prevention research (Philadelphia, Pa.). 3(8):910-6. 2010.
• He YQ, Sheng JQ, Ling XL. Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells. Experimental & molecular medicine. 44(12):723-32. 2012.