重组小鼠CD22蛋白

产品详情

  • 产品名称
    重组小鼠CD22蛋白
  • 蛋白种属
    小鼠
  • 表达宿主
    人胚胎肾细胞(HEK293)
  • Unitprot ID
  • 蛋白序列
    Met 1 - Arg 708
  • 克隆构建
    A DNA sequence encoding the mouse CD22 (BAE33829.1)(Met1-Arg708) was expressed with the Fc region of human IgG1 at the C-terminus.
  • 蛋白纯度
    > 95 % as determined by SDS-PAGE
  • 分子量
    The recombinant mouse CD22/Fc is a disulfide-linked homodimer. The reduced monomer comprises 931 amino acids and has a predicted molecular mass of 104.3 kDa. The apparent molecular mass of the protein is approximately 125 kDa in SDS-PAGE under reducing conditions.
  • 蛋白别名
    SIGLEC-2; CD22; LYB8; A530093D23; FLJ22814; MGC130020; SIGLEC2; LYB-8
  • 蛋白全名
    Cd22 protein
  • 蛋白描述
    CD22 is a member of the immunoglobulin superfamily, SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions, signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow, blood, and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR), CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors, the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR, and this in turn is governed by the binding of its extracellular domain to alpha2,6-linked sialic acid ligands. However, genetic studies in mice reveal that some CD22 functions are regulated by ligand binding, whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity, while CD22 inhibits CD19 phosphorylation via SHP-1.
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4.
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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