- Unitprot ID:O88947
A DNA sequence encoding the Mouse F10 Gly21-Asn481 is expressed with a 6His tag at the C-terminus.
>95% as determined by SDS-PAGE.
coagulation factor X(F10)
Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.
Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl and 1mM CaCl2 pH7.5..
<1.0 EU per μg protein as determined by the LAL method.
Lyophilized protein should be stored at <-20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days.