重组小鼠FCGRT蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Ser 297

A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified

> 95 % as determined by SDS-PAGE

Measured by its ability to bind human IgG1 in functional ELISA.

The recombinant heterodimer of human FCGRT / B2M comprises 385 ( 285 + 100 ) amino acids and has a calculated molecular mass of 43.5 ( 31.8 + 11.7 ) kDa. The apparent molecular mass of rh FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions

FCRN

IgG receptor FcRn large subunit p51

Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.