重组小鼠LIF蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Phe 203

A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified.

> 95 % as determined by SDS-PAGE

Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.
The ED₅₀ for this effect is typically 0.1-0.5 ng/mL.

The recombinant mouse LIF consists of 180 amino acids and predicts a molecular mass of 19.9 kDa. It migrates as approximately 34.1 kDa band in SDS-PAGE under reducing conditions.

RP23-329P17.1; HILDA; MLPLI; CDF; OTTMUSP00000005253; D FACTOR; DIA

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin.

Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.