重组小鼠LIF蛋白
- 蛋白种属:小鼠
- 表达宿主:人胚胎肾细胞(HEK293)
- Unitprot ID:P09056
产品详情
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产品名称
重组小鼠LIF蛋白
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蛋白种属
小鼠
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表达宿主
人胚胎肾细胞(HEK293)
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Unitprot ID
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蛋白序列
Met 1 - Phe 203
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克隆构建
A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified.
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蛋白纯度
> 95 % as determined by SDS-PAGE
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蛋白活性
Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.
The ED50 for this effect is typically 0.1-0.5 ng/mL.
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分子量
The recombinant mouse LIF consists of 180 amino acids and predicts a molecular mass of 19.9 kDa. It migrates as approximately 34.1 kDa band in SDS-PAGE under reducing conditions.
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蛋白别名
RP23-329P17.1; HILDA; MLPLI; CDF; OTTMUSP00000005253; D FACTOR; DIA
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蛋白描述
Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin.
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发货形式
Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!