- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IF
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
-
产品名称
MYLK antibody
-
抗体类型
多克隆
-
抗体来源
兔
-
抗体亚型
兔IgG
-
抗体描述
MYLK Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive WB detected in mouse uterus tissue, mouse large intestine tissue. Observed molecular weight by Western-blot: 135kd
-
抗体应用
ELISA, WB, IF
-
应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
-
特异性
Human,Mouse,Rat; other species not tested.
-
蛋白别名
DKFZp686I10125, FLJ12216, Kinase related protein, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK, MYLK1, myosin light chain kinase, smMLCK, Telokin
-
制备方法
This antibody was obtained by immunization of MYLK recombinant protein (Accession Number: BC113456). Purification method: Antigen affinity purified.
-
组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
储存方法
Store at -20℃. DO NOT ALIQUOT
-
背景介绍
MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria(PMID:20404178). The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles. It has 8 isoforms produced by alternative splicing and alternative initiation and our anti-MYLK antibody can recognize these isforms.
-
参考文献
- Sumagin R, Robin AZ, Nusrat A, Parkos CA. Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment. Mucosal immunology. 7(4):905-15. 2014.