- 抗体类型:单克隆
- 抗体来源:小鼠
- 抗体应用:ELISA, WB, IHC, IF
- 特异性:Human, Mouse, Rat; other species not tested.
产品详情
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产品名称
Nestin antibody
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抗体类型
单克隆
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抗体来源
小鼠
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抗体亚型
小鼠IgG2b
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抗体描述
Nestin Mouse Monoclonal antibody. Positive IF detected in U-251 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in Neuro-2a cells, HEK-293 cells, rat brain tissue. Observed molecular weight by Western-blot: 150-170 kDa
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抗体应用
ELISA, WB, IHC, IF
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human, Mouse, Rat; other species not tested.
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蛋白别名
NES, Nestin
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制备方法
This antibody was obtained by immunization of Peptide (Accession Number: NM_006617). Purification method: Protein A purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.