- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IF, IHC
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
PTBP1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
PTBP1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, BxPC-3 cells, HeLa cells, human lung tissue, K-562 cells, mouse lung tissue, rat heart tissue. Positive IHC detected in human prostate cancer tissue, human pancreas tissue. Positive IF detected in A2780 cells. Observed molecular weight by Western-blot: 59.6kd
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抗体应用
ELISA, WB, IF, IHC
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应用推荐
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1
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制备方法
This antibody was obtained by immunization of PTBP1 recombinant protein (Accession Number: NM_002819). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10
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参考文献
- Guo R, Li Y, Ning J, Sun D, Lin L, Liu X. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PloS one. 8(4):e62729. 2013.
- Gallagher IJ, Scheele C, Keller P. Integration of microRNA changes in vivo identifies novel molecular features of muscle insulin resistance in type 2 diabetes. Genome medicine. 2(2):9. 2010.
- Markus MA, Marques FZ, Morris BJ. Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs. PloS one. 6(12):e28926. 2011.