重组人PKM蛋白

人

大肠杆菌

Ser 2 - Pro 531

A DNA sequence encoding the human PKM2 isoform M2 (P14618-1) (Ser 2-Pro 531) was expressed, with a polyhistide tag at the N-terminus.

> 90 % as determined by SDS-PAGE

Kinase activity untested

The recombinant human PKM2 consisting of 541 amino acids and migrates as an approximately 59 kDa band in SDS-PAGE under reducing conditions as predicted.

CTHBP; THBP1; AL024370; AL024424; PK-2; HEL-S-30; MGC3932; OIP3; AA414905; TCB; PKM2; PKM; PK3; PK-3

Pyruvate kinase PKM

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Supplied as sterile PBS, pH 7.0, 10% glycerol
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

Please contact us for more information.

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.