重组大鼠FCGR3A蛋白

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  • 蛋白种属:大鼠
  • 表达宿主:人胚胎肾细胞(HEK293)
  • Unitprot ID:

产品详情

  • 产品名称
    重组大鼠FCGR3A蛋白
  • 蛋白种属
    大鼠
  • 表达宿主
    人胚胎肾细胞(HEK293)
  • Unitprot ID
  • 蛋白序列
    Met 1 - Pro 200
  • 克隆构建
    A DNA sequence encoding the rat FCGR3A (ABO70343.1) extracellular domain (Met 1-Pro 200) was expressed, fused with a polyhistidine tag at the C-terminus.
  • 蛋白纯度
    > 97 % as determined by SDS-PAGE
  • 蛋白活性
    Measured by its binding ability in a functional ELISA.
    Immobilized rat FCGR3A-His (Cat:504601) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 59.0-139.0 ng/ml.
  • 分子量
    The recombinant rat FCGR3A comprises 190 amino acids and predicts a molecular mass of 21.7 kDa. The apparent molecular mass of the rat FCGR3A is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
  • 蛋白别名
    RP11-5K23.1; CD16; FCRIIIA; FCRIII; FCGRIV; FCR-10; CD16A; FCGAMMARIV; 4833442P21RIK; IMD20; FCGR3; FCGR3A; FCGRIII; IGFR3; CD16-2; FCRL3; FCG3
  • 蛋白描述
    The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.\CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. \
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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