重组大鼠FCGR3A蛋白
- 蛋白种属:大鼠
- 表达宿主:人胚胎肾细胞(HEK293)
- Unitprot ID:
产品详情
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产品名称
重组大鼠FCGR3A蛋白
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蛋白种属
大鼠
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表达宿主
人胚胎肾细胞(HEK293)
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Unitprot ID
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蛋白序列
Met 1 - Pro 200
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克隆构建
A DNA sequence encoding the rat FCGR3A (ABO70343.1) extracellular domain (Met 1-Pro 200) was expressed, fused with a polyhistidine tag at the C-terminus.
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蛋白纯度
> 97 % as determined by SDS-PAGE
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蛋白活性
Measured by its binding ability in a functional ELISA.
Immobilized rat FCGR3A-His (Cat:504601) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 59.0-139.0 ng/ml.
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分子量
The recombinant rat FCGR3A comprises 190 amino acids and predicts a molecular mass of 21.7 kDa. The apparent molecular mass of the rat FCGR3A is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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蛋白别名
RP11-5K23.1; CD16; FCRIIIA; FCRIII; FCGRIV; FCR-10; CD16A; FCGAMMARIV; 4833442P21RIK; IMD20; FCGR3; FCGR3A; FCGRIII; IGFR3; CD16-2; FCRL3; FCG3
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蛋白描述
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.\CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. \
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发货形式
Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!