重组大鼠TDGF1蛋白

大鼠

人胚胎肾细胞（HEK293）

Met 1 - Cys 143

A DNA sequence encoding the rat TDGF1 (XP_001056317.2) (Met1-Cys143) was expressed with a polyhistidine tag at the C-terminus.

(32.6+48.9) % as determined by SDS-PAGE

Measured by its ability to bind with rat ACVR1B-Fc (Cat:504742) in a functional ELISA.

The recombinant rat TDGF1 comprises 138 amino acids and predicts a molecular mass of 15.5 kDa. The apparent molecular mass of the recombinant protein is approximately 22 and 19 kDa in SDS-PAGE under reducing conditions.

CR; TDGF1; CR1; CRIPTO; CRGF

Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.

Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.