重组小鼠REN蛋白

小鼠

人胚胎肾细胞（HEK293）

Met 1 - Arg 402

A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.

> 97 % as determined by SDS-PAGE

1. Measured by its ability to cleave the fluorogenic peptide substrate 5­FAM/QXL™ 520 (Peti­Peterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg.
2. Immobilized mouse REN1-His (Cat: 502823) at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 501652) with a linear range of 31.25-250 ng/ml.

The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.

REN1D; REN-A; RN-1; FLJ10761; REN; HNFJ2; D19352; RNR; REN1C; RENIN; REN-1

Renin-1

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.