重组人REN蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Arg 406

A DNA sequence encoding the human Renin (NP_000528.1) precursor (Met 1-Arg 406) was expressed with a polyhistidine tag at the C-terminus.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human RENIN-His (Cat: 501755) at 10 μg/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 501652) with a linear range of 0.156-5.0 μg/ml.

The secreted recombinant human Renin is a monomeric protein after cleavage of the signal peptide. The pro form consists of 394 amino acids and predictes a molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.

REN-A; RN-1; FLJ10761; REN; REN1D; RENIN; REN-1; RNR; REN1C; HNFJ2; D19352

Renin

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Lyophilized from sterile 25mM MES, 150mM NaCl, pH6.5
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.