- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IP, IHC
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
SHARPIN antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
SHARPIN Rabbit Polyclonal antibody. Positive WB detected in Raji cells, A549 cells, Jurkat cells. Positive IP detected in Raji cells. Positive IHC detected in human small intestine tissue. Observed molecular weight by Western-blot: 40kd
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抗体应用
ELISA, WB, IP, IHC
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
DKFZp434N1923, hSIPL1, SHARPIN, SIPL1
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制备方法
This antibody was obtained by immunization of SHARPIN recombinant protein (Accession Number: XM_017013888). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.
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参考文献
- Rickard JA, Anderton H, Etemadi N. TNFR1-dependent cell death drives inflammation in Sharpin-deficient mice. eLife. 3:. 2014.
- Boisson B, Laplantine E, Dobbs K. Human HOIP and LUBAC deficiency underlies autoinflammation, immunodeficiency, amylopectinosis, and lymphangiectasia. The Journal of experimental medicine. 212(6):939-51. 2015.
- de Almagro MC, Goncharov T, Newton K, Vucic D. Cellular IAP proteins and LUBAC differentially regulate necrosome-associated RIP1 ubiquitination. Cell death & disease. 6:e1800. 2015.
- Peltzer N, Rieser E, Taraborrelli L. HOIP deficiency causes embryonic lethality by aberrant TNFR1-mediated endothelial cell death. Cell reports. 9(1):153-65. 2014.
- Damgaard RB, Nachbur U, Yabal M. The ubiquitin ligase XIAP recruits LUBAC for NOD2 signaling in inflammation and innate immunity. Molecular cell. 46(6):746-58. 2012.
- Douglas T, Champagne C, Morizot A, Lapointe JM, Saleh M. The Inflammatory Caspases-1 and -11 Mediate the Pathogenesis of Dermatitis in Sharpin-Deficient Mice. Journal of immunology (Baltimore, Md. : 1950). 195(5):2365-73. 2015.
- Boisson B, Laplantine E, Prando C. Immunodeficiency, autoinflammation and amylopectinosis in humans with inherited HOIL-1 and LUBAC deficiency. Nature immunology. 13(12):1178-86. 2012.
- Keusekotten K, Elliott PR, Glockner L. OTULIN antagonizes LUBAC signaling by specifically hydrolyzing Met1-linked polyubiquitin. Cell. 153(6):1312-26. 2013.