- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IF, IP, WB, FC
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
Spartin, SPG20 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Spartin, SPG20 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in NIH/3T3 cells, HEK-293 cells, HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue, SH-SY5Y cells. Positive FC detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75 kDa,84 kDa
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抗体应用
ELISA, IHC, IF, IP, WB, FC
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
KIAA0610, SPARTIN, Spastic paraplegia 20 protein, SPG20, TAHCCP1
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制备方法
This antibody was obtained by immunization of Spartin, SPG20 recombinant protein (Accession Number: NM_015087). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer paitents, may play a role in cytokinesis arrest in colorectal tumorigenesis.
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参考文献
- Renvoisé B, Stadler J, Singh R, Bakowska JC, Blackstone C. Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling. Human molecular genetics. 21(16):3604-18. 2012.
- Karlsson AB, Washington J, Dimitrova V, Hooper C, Shekhtman A, Bakowska JC. The role of spartin and its novel ubiquitin binding region in DALIS occurrence. Molecular biology of the cell. 25(8):1355-65. 2014.
- Milewska M, Byrne PC. Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells. Cell biology international. 39(9):1007-15. 2015.
- Nicholson JM, Macedo JC, Mattingly AJ. Chromosome mis-segregation and cytokinesis failure in trisomic human cells. eLife. 4:. 2015.
- Yan J, Zhang H, Liu Y. Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice. Journal of cell science. 129(3):492-501. 2016.
- Manzini MC, Rajab A, Maynard TM. Developmental and degenerative features in a complicated spastic paraplegia. Annals of neurology. 67(4):516-25. 2010.
- Lind GE, Raiborg C, Danielsen SA. SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis. Oncogene. 30(37):3967-78. 2011.
- Urbanczyk A, Enz R. Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets. Journal of neurochemistry. 118(5):737-48. 2011.