重组小鼠CD4蛋白

产品详情

  • 产品名称
    重组小鼠CD4蛋白
  • 蛋白种属
    小鼠
  • 表达宿主
    人胚胎肾细胞（HEK293）
  • Unitprot ID
    Q3TSV7
  • 蛋白序列
    Met 1 - Thr 394
  • 克隆构建
    A DNA sequence encoding the extracellular domain of mouse CD4 (NP_038516.1) (Met 1-Thr 394) was expressed, fused with a polyhistidine tag at the C-terminus.
  • 蛋白纯度
    > 95 % as determined by SDS-PAGE
  • 蛋白活性
    Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to mCD4-His coated plates (0.8μg/mL and 100μL/well), approximately >50% will adhere specifically after 30 minutes at 37℃.
  • 分子量
    The recombinant mouse CD4 consists of 379 amino acids and has a predicted molecular mass of 42.8 kDa. As a result of glycosylation, the apparent molecular mass of rmCD4 is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
  • 蛋白别名
    LY-4; CD4; L3T4; CD4MUT
  • 蛋白全名
    T-cell surface glycoprotein CD4
  • 蛋白描述
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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