Thymosin Alpha 1, purity >98% 多肽 (TFA已除)

Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN-OH

Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH

28

98.17

C₁₂₉H₂₁₅N₃₃O₅₅

3108.25

固相合成

Thymic Hormones and Their Role in Aging. The increase in the average life span of humans during the last century has been accompanied by a parallel increase in a number of diseases associated with aging, including cancer, and certain infectious and autoimmune diseases. The decline of immunocompetence with aging has been correlated with the increased incidence of these diseases and extensive reviews describing the changes in the immune system during the aging process has recently been published. Although the decline of the immune system is accompanied by alterations in all its compartments, the most affected appears to be the T-cell compartment. Decline in T cell mitogen-induced proliferation, allogeneic mixed leukocyte reaction (MLR), cytotoxic T lymphocyte responses (CTE), helper T-cell activity, IL2 production, IL2 receptor expression, calcium uptake, PNP activity (PNPpurine nucleoside phosphorylase), adenylate cyclase activity, etc have been observed in T-cells from aging individuals and animals. These changes lead to an increased susceptibility to infections, decrease resistance to tumor growth, and an increased incidence of autoimmune diseases, probably secondary to an immunologic imbalar~ce resulting in an increased response towards self antigens, characterized by a rise in the frequency of autoantibodies observed in aged individuals.

一般情况下， Thymosin Alpha 1, purity >98% 多肽 (TFA已除) 粉末应该保存在-20甚至-70摄氏度. Thymosin Alpha 1, purity >98% 多肽 (TFA已除) 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Sztein MB, Goldstein AL. Thymic hormones--a clinical update. Springer Semin Immunopathol. 1986;9(1):1-18. doi: 10.1007/BF00201901. PMID: 3014672.
• Dominari A, Hathaway Iii D, Pandav K, Matos W, Biswas S, Reddy G, Thevuthasan S, Khan MA, Mathew A, Makkar SS, Zaidi M, Fahem MMM, Beas R, Castaneda V, Paul T, Halpern J, Baralt D. Thymosin alpha 1: A comprehensive review of the literature. World J Virol. 2020 Dec 15;9(5):67-78. doi: 10.5501/wjv.v9.i5.67. PMID: 33362999; PMCID: PMC7747025.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。