- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IP, IF
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
TRIM21 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
TRIM21 Rabbit Polyclonal antibody. Positive WB detected in A549 cells, HeLa cells, HL-60 cells, HT-1080 cells, mouse pancreas tissue. Positive IP detected in A549 cells. Positive IHC detected in human lung cancer tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 52 kDa
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抗体应用
ELISA, WB, IHC, IP, IF
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应用推荐
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
52 kDa Ro protein, RING finger protein 81, RNF81, Ro(SS A), RO52, SS A, SSA, SSA1, TRIM21, tripartite motif containing 21
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制备方法
This antibody was obtained by immunization of TRIM21 recombinant protein (Accession Number: NM_003141). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.
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参考文献
- Shibata N, Ohoka N, Sugaki Y. Degradation of Stop Codon Read-through Mutant Proteins via the Ubiquitin-Proteasome System Causes Hereditary Disorders. The Journal of biological chemistry. 290(47):28428-37. 2015.
- Fan W, Zhang D, Qian P. Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism. Antiviral research. 127:32-40. 2016.