The buffer is one of the most popular currently used, and is commonly employed in molecular and cell biology, chemistry, and material science, among many others.
配制L Sodium Phosphate Buffer
M表 1. 配方
| 试剂名称 | 试剂量 | 浓度 | 分子式/分子量 | CAS号 |
|---|---|---|---|---|
| 2.231 g | 0.018 M | NaH2PO4/119.98 | 7558-80-7 | |
| 11.555 g | 0.081 M | Na2HPO4/141.96 | 7558-79-4 |
- 在合适的容器中装入 0.8 L 的蒸馏水.
- 往溶液中加入 2.231 g 的 磷酸二氢钠.
- 往溶液中加入 11.555 g 的 磷酸氢二钠.
- 加入 蒸馏水 直到体积为 1 L.
- Phosphate buffer is highly water soluble and has a high buffering capacity, but will inhibit enzymatic activity and precipitates in ethanol.