载体基本信息 | |||
载体名称 | pBridge | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6527 bp | 载体类型 | Yeast Plasmids |
载体来源 | Clontech | 拷贝数 | High copy number |
pBridge 载体载体图谱
pBridge 载体载体序列
LOCUS Exported 6527 bp ds-DNA circular SYN 11-1-2013 DEFINITION Yeast three-hybrid vector for expressing a ""bait"" protein fused to the GAL4 DNA-binding domain, plus an additional bridge protein. ACCESSION . VERSION . KEYWORDS pBridge SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6527) AUTHORS Clontech TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..6527 /organism="synthetic DNA construct" /lab_host="Saccharomyces cerevisiae" /mol_type="other DNA" promoter 10..406 /gene="S. cerevisiae ADH1" /note="ADH1 promoter" /note="promoter for alcohol dehydrogenase 1" CDS 434..874 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /note="GAL4 DNA binding domain" /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" misc_feature 878..905 /label="MCS I" /note="MCS 1" /note="multiple cloning site" terminator 921..1108 /gene="S. cerevisiae ADH1" /note="ADH1 terminator" /note="transcription terminator for alcohol dehydrogenase 1" CDS complement(1161..1835) /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /note="TRP1" /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" promoter complement(1836..1937) /gene="S. cerevisiae TRP1" /note="TRP1 promoter" promoter 2259..2609 /gene="S. cerevisiae MET17" /gene_synonym="MET15; MET25" /note="MET17 promoter" /note="expression is repressed by methionine" CDS 2619..2621 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 2631..2657 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" CDS 2679..2699 /codon_start=1 /locus_tag=" " /product="nuclear localization signal of SV40 large T antigen" /note="SV40 NLS" /note=" " /protein_id=" " /translation="PKKKRKV" misc_feature 2700..2723 /label="MCS II" /note="MCS 2" /note="multiple cloning site" terminator 2825..3095 /gene="S. cerevisae PGK1" /note="PGK1 terminator" /note="transcription terminator for 3-phosphoglycerate kinase gene" rep_origin complement(3357..3945) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4116..4976) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4977..5081) /gene="bla" /note="AmpR promoter" rep_origin 5363..6527 /note="2u ori" /note="yeast 2u plasmid origin of replication"
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