下载GeneBank文件(.gb)

LOCUS       pBridge.        6527 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast three-hybrid vector for expressing a """"bait"""" protein 
            fused to the GAL4 DNA-binding domain, plus an additional bridge 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    pBridge.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..905
                     /label=MCS I
                     /note="MCS 1"
                     /note="multiple cloning site"
     terminator      921..1108
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(1164..1835)
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     promoter        complement(1836..1937)
                     /label=TRP1 promoter
     primer_bind     complement(1951..1967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1975..1991)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1999..2029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2044..2065)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2259..2609
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     CDS             2619..2621
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2631..2657
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2679..2699
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    2700..2723
                     /label=MCS II
                     /note="MCS 2"
                     /note="multiple cloning site"
     terminator      2825..3095
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     rep_origin      complement(3357..3945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4119..4976)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4977..5081)
                     /label=AmpR promoter
     rep_origin      5363..6527
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"