载体基本信息 | |||
载体名称 | pQE-30 UA | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3504 bp | 载体类型 | Qiagen Vectors |
载体来源 | Qiagen | 拷贝数 | High copy number |
pQE-30 UA 载体载体图谱
pQE-30 UA 载体载体序列
LOCUS Exported 3504 bp ds-DNA circular SYN 09-1-2013 DEFINITION Bacterial expression and 6xHis-tagging vector for UA cloning of a PCR product. ACCESSION . VERSION . KEYWORDS pQE-30 UA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3504) AUTHORS Qiagen TITLE Direct Submission COMMENT This vector is supplied in a linearized form, with bases 174-175 replaced with 3'-U overhangs. FEATURES Location/Qualifiers source 1..3504 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 10..54 /note="T5 promoter" /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 30..46 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 62..78 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 101..106 /note="ribosome binding site" CDS 115..117 /codon_start=1 /product="start codon" /note="ATG" /translation="M" CDS 127..144 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 145..235 /note="MCS" /note="multiple cloning site" misc_feature 235..245 /note="stop codons" /note="stop codons in all three reading frames" terminator 251..345 /gene=" " /note="lambda t0 terminator" /note="transcription terminator from phage lambda" CDS 389..1048 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" terminator 1113..1199 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin complement(1680..2268) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2439..3299) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3300..3404) /gene="bla" /note="AmpR promoter"
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