pQE-10 载体 (V010810#)

载体名称 pQE-10 载体抗性 Ampicillin
载体长度 3442 bp 载体类型 Qiagen Vectors
载体来源 Qiagen 拷贝数 High copy number

pQE-10 载体载体图谱

pQE-103442 bp6001200180024003000T5 promoterlac operatorRBSATG6xHisMCSstop codonslambda t0 terminatorCmRrrnB T1 terminatorbomoriAmpRAmpR promoter

pQE-10 载体载体序列

LOCUS       pQE-10.        3442 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing N-terminally 6xHis-tagged proteins. 
            For other reading frames, use pQE-9 or pQE-11.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3442)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Discontinued pQE vector.
FEATURES             Location/Qualifiers
     source          1..3442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /product="start codon"
                     /label=start codon
     CDS             127..144
                     /note="6xHis affinity tag"
     misc_feature    147..172
                     /note="multiple cloning site"
     misc_feature    172..182
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      188..282
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             326..982
                     /note="chloramphenicol acetyltransferase"
     terminator      1050..1136
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
     misc_feature    1292..1432
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1618..2206)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(2380..3237)
     promoter        complement(3238..3342)
                     /label=AmpR promoter