载体基本信息 | |||
载体名称 | pIX 3.0 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2697 bp | 载体类型 | Qiagen Vectors |
载体来源 | Qiagen | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pIX 3.0 载体载体图谱
pIX 3.0 载体载体序列
LOCUS Exported 2697 bp ds-DNA circular SYN 09-1-2013 DEFINITION Prokaryotic in vitro translation vector for cloning PCR products generated with the EasyXpress Linear Template Kit. ACCESSION . VERSION . KEYWORDS pIX 3.0 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2697) AUTHORS Qiagen TITLE Direct Submission COMMENT The PCR products include regulatory elements for expression in bacterial extracts, plus optional tag sequences. FEATURES Location/Qualifiers source 1..2697 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" primer_bind 379..395 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 406..461 /note="MCS" /note="multiple cloning site" primer_bind complement(476..492) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 500..516 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(524..554) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(878..1466) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1637..2497) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2498..2602) /gene="bla" /note="AmpR promoter"
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