pIX 3.0 载体 (V010812#)

载体基本信息
载体名称 pIX 3.0 载体抗性 Ampicillin
载体长度 2697 bp 载体类型 Qiagen Vectors
载体来源 Qiagen 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pIX 3.0 载体载体图谱

pIX 3.02697 bp600120018002400M13 fwdMCSM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

pIX 3.0 载体载体序列

LOCUS       pIX_3.0.        2697 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Prokaryotic in vitro translation vector for cloning PCR products 
            generated with the EasyXpress Linear Template Kit.
ACCESSION   .
VERSION     .
KEYWORDS    pIX 3.0.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The PCR products include regulatory elements for expression in 
            bacterial extracts, plus optional tag sequences.
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    406..461
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(476..492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(500..516)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(524..554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(569..590)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(878..1466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1640..2497)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2498..2602)
                     /label=AmpR promoter

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