pIX 3.0 载体 (V010812#)

载体基本信息
载体名称 pIX 3.0 载体抗性 Ampicillin
载体长度 2697 bp 载体类型 Qiagen Vectors
载体来源 Qiagen 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pIX 3.0 载体载体图谱

pIX 3.02697 bp600120018002400M13 fwdMCSM13 revlac operatorlac promoteroriAmpRAmpR promoter

pIX 3.0 载体载体序列

LOCUS       Exported                2697 bp ds-DNA    circular SYN 09-1-2013
DEFINITION  Prokaryotic in vitro translation vector for cloning PCR products 
            generated with the EasyXpress Linear Template Kit.
ACCESSION   .
VERSION     .
KEYWORDS    pIX 3.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Qiagen
  TITLE     Direct Submission
COMMENT     The PCR products include regulatory elements for expression in 
            bacterial extracts, plus optional tag sequences.
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    406..461
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(476..492)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    500..516
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(524..554)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(878..1466)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1637..2497)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2498..2602)
                     /gene="bla"
                     /note="AmpR promoter"

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