载体基本信息 | |||
载体名称 | pET-15b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5708 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-15b 载体载体图谱
pET-15b 载体载体序列
LOCUS Exported 5708 bp ds-DNA circular SYN 01-12-2013 DEFINITION Bacterial vector for inducible expression of N-terminally 6xHis-tagged proteins. ACCESSION . VERSION . KEYWORDS pET-15b SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5708) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..5708 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" terminator 213..260 /note="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_feature 319..335 /note="MCS" /note="multiple cloning site" CDS complement(336..353) /codon_start=1 /product="thrombin recognition and cleavage site" /note="thrombin site" /translation="LVPRGS" CDS complement(363..380) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" CDS complement(390..392) /codon_start=1 /product="start codon" /note="ATG" /translation="M" RBS 400..404 /note="ribosome binding site" protein_bind 436..460 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(461..479) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" promoter 788..865 /gene="lacI" /note="lacI promoter" /note=" " CDS 866..1948 /codon_start=1 /gene="lacI" /product="lac repressor" /note="lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" CDS 3260..3451 /codon_start=1 /gene="rop" /product="Rop protein" /note="rop" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" rep_origin complement(3881..4469) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4640..5500) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5501..5605) /gene="bla" /note="AmpR promoter"
我们不提供该载体,载体信息仅供查询和参考。