载体基本信息 | |||
载体名称 | pET-11b | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 5676 bp | 载体类型 | pET & Duet Vectors (Novagen) |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pET-11b 载体载体图谱
pET-11b 载体载体序列
LOCUS Exported 5676 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Bacterial expression vector pET-11b, complete sequence. ACCESSION LT727490 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5676) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5676) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5676) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5676 /organism="Bacterial expression vector pET-11b" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 2291." /db_xref="taxon:1944664" promoter 10..38 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" regulatory complement(213..260) /regulatory_class="terminator" /note="T7T" terminator 213..260 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(327..359) /codon_start=1 /note="unnamed protein product; T710f" /protein_id="SJL88637.1" /translation="MASMTGGQQMG" misc_feature complement(360..404) /label=5UTR /note="5UTR" RBS 367..389 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind 404..428 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." regulatory complement(406..447) /regulatory_class="promoter" /note="T7lac" promoter complement(429..447) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 756..833 /regulatory_class="promoter" /note="lacI" promoter 756..833 /gene="lacI" /label=lacI promoter CDS 834..1916 /codon_start=1 /note="unnamed protein product; lacI" /protein_id="SJL88638.1" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" CDS 3228..3419 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature 3521..3663 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3665..4066) /direction=LEFT /label=pMB1 ori /note="pMB1 ori" rep_origin complement(3849..4437) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4608..5468) /codon_start=1 /note="unnamed protein product; ampicillin resistance" /protein_id="SJL88639.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5469..5573) /gene="bla" /label=AmpR promoter
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