载体基本信息 | |||
载体名称 | pCBG99-Basic | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 4793 bp | 载体类型 | Luciferase Vectors |
载体来源 | Promega | 拷贝数 | High copy number |
pCBG99-Basic 载体载体图谱
pCBG99-Basic 载体载体序列
LOCUS Exported 4793 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Luciferase reporter vector pCBG99-Basic, complete sequence. ACCESSION AY258595 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4793) AUTHORS Almond B, Chauvin F. TITLE Chroma-Luc? Series Reporter Vectors Technical Manual #TM061 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4793) AUTHORS Almond B, Chauvin F. TITLE Direct Submission JOURNAL Submitted (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4793) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4793 /organism="Luciferase reporter vector pCBG99-Basic" /mol_type="other DNA" /db_xref="taxon:236848" misc_feature 1..58 /label=multiple cloning region /note="multiple cloning region" regulatory 82..91 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 88..1716 /codon_start=1 /product="CBG99luc" /label=CBG99luc /protein_id="AAP83311.1" /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT ESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD SIPRNVTGKITRKELLKQLLEKAGG" RBS 1038..1046 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" misc_feature 1747..1968 /label=SV40 late poly A /note="SV40 late poly A" polyA_signal 1756..1877 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2293 /label=ColE1-derived /note="ColE1-derived" rep_origin complement(2296..2884) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3055..3915) /codon_start=1 /gene="ampR" /product="beta-lactamase" /label=ampR /note="AmpR" /protein_id="AAP83310.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(3055..3915) /gene="ampR" /label=ampR promoter complement(3916..4020) /gene="bla" /label=AmpR promoter rep_origin 4047..4502 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" misc_feature 4048..4502 /label=f1 origin /note="f1 origin" misc_feature 4633..4786 /label=upstream poly A /note="upstream poly A" polyA_signal 4633..4681 /note="synthetic polyadenylation signal" misc_feature 4695..4786 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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