载体基本信息 | |||
载体名称 | pBACgus-2cp | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7560 bp | 载体类型 | Insect Cell Vectors |
载体来源 | Novagen (EMD Millipore) | 拷贝数 | High copy number |
pBACgus-2cp 载体载体图谱
pBACgus-2cp 载体载体序列
LOCUS pBACgus-2cp. 7560 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid with the polyhedrin promoter, encoding beta-glucuronidase plus an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette. ACCESSION . VERSION . KEYWORDS pBACgus-2cp. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7560) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 7560) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..7560 /mol_type="other DNA" /organism="synthetic DNA construct" misc_recomb 329..1156 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" CDS complement(1170..2978) /label=GUS /note="beta-glucuronidase" promoter complement(2987..3302) /label=p6.9 promoter /note="baculovirus late basic protein promoter" promoter 3317..3408 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 3415..3417 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 3421..3438 /label=6xHis /note="6xHis affinity tag" CDS 3448..3465 /label=thrombin site /note="thrombin recognition and cleavage site" CDS 3469..3513 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS 3529..3543 /label=enterokinase site /note="enterokinase recognition and cleavage site" misc_feature 3546..3609 /label=MCS /note="MCS" /note="multiple cloning site" CDS 3610..3627 /label=6xHis /note="6xHis affinity tag" misc_recomb 3664..5021 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" rep_origin complement(5284..5872) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6046..6903) /label=AmpR /note="beta-lactamase" promoter complement(6904..7008) /label=AmpR promoter rep_origin 7035..7490 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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