pcDNAII 载体 (V011746#)

载体基本信息
载体名称 pcDNAII 载体抗性 Ampicillin
载体长度 3013 bp 载体类型 I.M.A.G.E. Consortium Plasmids
载体来源 Invitrogen (Life Technologies) 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pcDNAII 载体载体图谱

pcDNAII3013 bp6001200180024003000MCSSP6 promoterM13 revlac operatorlac promoteroriAmpRAmpR promoterf1 oriM13 fwd

pcDNAII 载体载体序列

LOCUS       Exported                3013 bp ds-DNA    circular SYN 28-2-2013
DEFINITION  Bacterial vector with an extensive polylinker for the cloning and 
            expression of cDNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNAII
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    10..122
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(134..152)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(170..186)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    194..210
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(218..248)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(572..1160)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1331..2191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance fo ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2192..2296)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(2377..2832)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     CDS             complement(2582..174)
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSYLGDTIEYSSYASSLVPSSDPLVTAASVLEFCRYPSHW
                     RPLEHASRGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGF
                     PRQALNRGLPLGFRFRALRHLDRKKLDLGDGSRSGPSP"
     primer_bind     2974..2990
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2997..2
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"

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