载体基本信息 | |||
载体名称 | pcDNAII | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3013 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | Invitrogen (Life Technologies) | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pcDNAII 载体载体图谱
pcDNAII 载体载体序列
LOCUS Exported 3013 bp ds-DNA circular SYN 28-2-2013 DEFINITION Bacterial vector with an extensive polylinker for the cloning and expression of cDNAs. ACCESSION . VERSION . KEYWORDS pcDNAII SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3013) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission FEATURES Location/Qualifiers source 1..3013 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" misc_feature 10..122 /note="MCS" /note="multiple cloning site" promoter complement(134..152) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(170..186) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 194..210 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(218..248) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(572..1160) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1331..2191) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance fo ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2192..2296) /gene="bla" /note="AmpR promoter" rep_origin complement(2377..2832) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(2582..174) /codon_start=1 /gene="lacZ (fragment)" /product="LacZ-alpha fragment of beta-galactosidase" /note="lacZ-alpha" /translation="MTMITPSYLGDTIEYSSYASSLVPSSDPLVTAASVLEFCRYPSHW RPLEHASRGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART DRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAGF PRQALNRGLPLGFRFRALRHLDRKKLDLGDGSRSGPSP" primer_bind 2974..2990 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 2997..2 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase"
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