载体基本信息 | |||
载体名称 | pBluescriptR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2998 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescriptR 载体载体图谱
pBluescriptR 载体载体序列
LOCUS pBluescriptR. 2998 bp DNA circular SYN 01-JAN-1980 DEFINITION pBluescript derivative modified at RIKEN for cDNA cloning. ACCESSION . VERSION . KEYWORDS pBluescriptR. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2998) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission REFERENCE 2 (bases 1 to 2998) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2998 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 4..459 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 623..641 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(674..707) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." misc_feature 733..798 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(810..828) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(849..865) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(873..889) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(897..927) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(942..963) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1251..1839) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2013..2870) /label=AmpR /note="beta-lactamase" promoter complement(2871..2975) /label=AmpR promoter
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