pBluescriptR 载体 (V011752#)

载体基本信息
载体名称 pBluescriptR 载体抗性 Ampicillin
载体长度 2998 bp 载体类型 I.M.A.G.E. Consortium Plasmids
载体来源 I.M.A.G.E. Consortium 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

pBluescriptR 载体载体图谱

pBluescriptR2998 bp600120018002400f1 oriM13 fwdT7 promoterloxPMCST3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

pBluescriptR 载体载体序列

LOCUS       pBluescriptR.        2998 bp DNA     circular SYN 01-JAN-1980
DEFINITION  pBluescript derivative modified at RIKEN for cDNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    pBluescriptR.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(674..707)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    733..798
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(810..828)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(849..865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(873..889)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(897..927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(942..963)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1251..1839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2013..2870)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2871..2975)
                     /label=AmpR promoter

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