载体基本信息 | |||
载体名称 | pBluescriptR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2998 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
pBluescriptR 载体载体图谱
pBluescriptR 载体载体序列
LOCUS Exported 2998 bp ds-DNA circular SYN 27-2-2013 DEFINITION pBluescript derivative modified at RIKEN for cDNA cloning. ACCESSION . VERSION . KEYWORDS pBluescriptR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2998) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission FEATURES Location/Qualifiers source 1..2998 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" rep_origin 4..459 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 623..641 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 674..707 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." misc_feature 733..798 /note="MCS" /note="multiple cloning site" promoter complement(810..828) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(849..865) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 873..889 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(897..927) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(1251..1839) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2010..2870) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2871..2975) /gene="bla" /note="AmpR promoter"
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