Lafmid BA 载体 (V011764#)

载体基本信息
载体名称 Lafmid BA 载体抗性 Ampicillin
载体长度 3152 bp 载体类型 I.M.A.G.E. Consortium Plasmids
载体来源 I.M.A.G.E. Consortium 拷贝数 High copy number
5'引物 M13 fwd 3'引物 M13 rev

Lafmid BA 载体载体图谱

Lafmid BA3152 bp6001200180024003000f1 oriM13 fwdMCSM13 revlac operatorlac promoteroriAmpR

Lafmid BA 载体载体序列

LOCUS       Exported                3152 bp ds-DNA    circular SYN 26-2-2013
DEFINITION  Phagemid vector used for cDNA cloning.
ACCESSION   .
VERSION     .
KEYWORDS    Lafmid BA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     rep_origin      complement(100..555)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     1042..1058
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1070..1101
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(1114..1130)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1116..1118)
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     protein_bind    1138..1154
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1162..1192)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(1516..2104)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2275..3135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"

我们不提供该载体,载体信息仅供查询和参考。