载体基本信息 | |||
载体名称 | Lafmid BA | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3152 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
Lafmid BA 载体载体图谱
Lafmid BA 载体载体序列
LOCUS Lafmid_BA. 3152 bp DNA circular SYN 01-JAN-1980 DEFINITION Phagemid vector used for cDNA cloning. ACCESSION . VERSION . KEYWORDS Lafmid BA. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3152) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission REFERENCE 2 (bases 1 to 3152) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3152 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(42..555) /direction=LEFT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 1042..1058 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 1070..1101 /label=MCS /note="MCS" /note="multiple cloning site" primer_bind complement(1114..1130) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1138..1154) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1162..1192) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1207..1228) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1516..2104) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2278..3135) /label=AmpR /note="beta-lactamase"
我们不提供该载体,载体信息仅供查询和参考。