载体基本信息 | |||
载体名称 | Lafmid BA | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3152 bp | 载体类型 | I.M.A.G.E. Consortium Plasmids |
载体来源 | I.M.A.G.E. Consortium | 拷贝数 | High copy number |
5'引物 | M13 fwd | 3'引物 | M13 rev |
Lafmid BA 载体载体图谱
Lafmid BA 载体载体序列
LOCUS Exported 3152 bp ds-DNA circular SYN 26-2-2013 DEFINITION Phagemid vector used for cDNA cloning. ACCESSION . VERSION . KEYWORDS Lafmid BA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3152) AUTHORS I.M.A.G.E. Consortium TITLE Direct Submission FEATURES Location/Qualifiers source 1..3152 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" rep_origin complement(100..555) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 1042..1058 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" misc_feature 1070..1101 /note="MCS" /note="multiple cloning site" primer_bind complement(1114..1130) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS complement(1116..1118) /codon_start=1 /product="start codon" /note="ATG" /translation="M" protein_bind 1138..1154 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1162..1192) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(1516..2104) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2275..3135) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW"
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