载体基本信息 | |||
载体名称 | pLC291 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 7506 bp | 载体类型 | Expression vector |
载体来源 | Chubiz LM, Purswani J, Carroll SM, Marx CJ. |
pLC291 载体载体图谱
pLC291 载体载体序列
LOCUS Exported 7506 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pLC291, complete sequence. ACCESSION KC296705 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7506) AUTHORS Chubiz LM, Purswani J, Carroll SM, Marx CJ. TITLE A novel pair of inducible expression vectors for use in Methylobacterium extorquens JOURNAL BMC Res Notes 6, 183 (2013) PUBMED 23648175 REFERENCE 2 (bases 1 to 7506) AUTHORS Chubiz LM, Purswani J, Marx CJ. TITLE A novel set of broad-host, inducible expression vectors for use in Methylobacterium extorquens and other gram-negative bacteria JOURNAL Unpublished REFERENCE 3 (bases 1 to 7506) AUTHORS Chubiz LM, Purswani J, Marx CJ. TITLE Direct Submission JOURNAL Submitted (10-DEC-2012) Organsmic and Evolutionary Biology, Harvard University, 16 Divinity Ave., Cambridge, MA 02138, USA REFERENCE 4 (bases 1 to 7506) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7506 /organism="Expression vector pLC291" /mol_type="other DNA" /db_xref="taxon:1291081" rep_origin 4..712 /label=oriV /note="oriV" rep_origin 11..642 /label=oriV /note="incP origin of replication" rep_origin 1105..1693 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 1180..1735 /label=ColE1 /note="ColE1" protein_bind 1981..2002 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 2011..2053 /label=pLac /note="pLac" promoter 2017..2047 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2055..2071 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2079..2095 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 2131..2757 /codon_start=1 /gene="tetR" /product="TetR repressor" /label=tetR /note="derived from Tn10" /protein_id="AGE65910.1" /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" gene 2131..2757 /gene="tetR" /label=tetR CDS 2134..2757 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" primer_bind 2801..2817 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 2816..3279 /regulatory_class="terminator" /note="TrrnB" terminator 2999..3085 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3177..3204 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" misc_feature 3281..3362 /label=pR /note="pR" misc_feature 3370..3388 /label=tetO /note="tetO" protein_bind 3370..3388 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" CDS complement(3944..4759) /codon_start=1 /gene="kan" /product="aminoglycoside 3'-phosphotransferase" /label=kan /protein_id="AGE65909.1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene complement(3944..4759) /gene="kan" /label=kan CDS complement(5431..6579) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" misc_feature 6761..7499 /label=oriT /note="oriT" CDS complement(6851..7222) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 7255..7364 /note="incP origin of transfer"
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