载体基本信息 | |||
载体名称 | pME6031 | 载体抗性 | Tetracycline |
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载体长度 | 8310 bp | 载体类型 | Shuttle vector |
载体来源 | Heeb S, Itoh Y, Nishijyo T, Schnider U, Keel C, Wade J, Walsh U, O'Gara F, Haas D. |
pME6031 载体载体图谱
pME6031 载体载体序列
LOCUS Exported 8310 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector pME6031, complete sequence. ACCESSION AF118811 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8310) AUTHORS Heeb S, Itoh Y, Nishijyo T, Schnider U, Keel C, Wade J, Walsh U, O'Gara F, Haas D. TITLE Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria JOURNAL Mol. Plant Microbe Interact. 13 (2), 232-237 (2000) PUBMED 10659714 REFERENCE 2 (bases 1 to 8310) AUTHORS Heeb S. TITLE Direct Submission JOURNAL Submitted (30-DEC-1998) Laboratoire de Biologie Microbienne, University of Lausanne, Batiment de Biologie, Lausanne CH-1015, Switzerland REFERENCE 3 (bases 1 to 8310) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..8310 /organism="Shuttle vector pME6031" /host="Pseudomonas pVS1; Escherichia p15A replicons" /mol_type="other DNA" /label=derived from plasmids pACYC177, pVK100, and pME290 /note="derived from plasmids pACYC177, pVK100, and pME290" /db_xref="taxon:89145" CDS 195..881 /codon_start=1 /product="pVS1 resolvase" /label=pVS1 resolvase /note="ORF1; not required for stable maintenance" /protein_id="AAD19683.1" /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE RQEEQA" CDS 878..1093 /codon_start=1 /product="hypothetical protein" /label=hypothetical protein /note="ORF2; not required for stable maintenance" /protein_id="AAD19684.1" /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA GLSDAKLAAWPASLAAEETERRRLKR" CDS 1180..1809 /codon_start=1 /product="pVS1 partitioning protein" /label=pVS1 partitioning protein /note="StaA; similar to IncC2" /protein_id="AAD19685.1" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 1830..2045 /codon_start=1 /product="hypothetical protein" /label=hypothetical protein /note="ORF3; not required for stable maintenance" /protein_id="AAD19686.1" /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT KLKMYAVRQGKTITELLSEYIAQLPE" misc_feature 2075..2088 /label=KorB box /note="KorB box" CDS 2238..3311 /codon_start=1 /product="pVS1 replication protein" /label=pVS1 replication protein /note="RepA" /protein_id="AAD19687.1" /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3377..3571 /label=pVS1 origin of replication /note="pVS1 origin of replication" misc_feature 3377..3384 /label=DnaA box /note="DnaA box" repeat_region 3388..3471 /label=21 bp repeats /note="21 bp repeats" misc_feature 3472..3563 /label=AT-rich region /note="AT-rich region" misc_feature 3564..3571 /label=DnaA box /note="DnaA box" rep_origin 4264..5093 /label=p15A origin of replication /note="p15A origin of replication" rep_origin 4281..4826 /direction=RIGHT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." misc_feature 5068..5089 /label=p15A origin of transfer /note="p15A origin of transfer" misc_difference 5136..5414 /label=part of terminator absent in pME6030 /note="part of terminator absent in pME6030" regulatory 5148..5414 /regulatory_class="terminator" /note="T4 transcription terminator" misc_feature 5416..5526 /label=multiple cloning site /note="multiple cloning site" CDS complement(6315..6992) /codon_start=1 /product="RK2 tetracycline repressor protein" /label=RK2 tetracycline repressor protein /note="TetR" /protein_id="AAD19689.1" /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK GDD" CDS complement(6315..6965) /codon_start=1 /gene="tetR" /product="tetracycline resistance regulatory protein" /label=TetR /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD" CDS 6996..8270 /codon_start=1 /product="RK2 tetracycline resistance protein" /label=RK2 tetracycline resistance protein /note="TetA" /protein_id="AAD19688.1" /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG AALYLLCLPALRRGLWSGAGQRADR" CDS 7071..8270 /codon_start=1 /gene="tetA" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR "
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