我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pJLIC
- 载体抗性:
- Ampicillin
- 载体长度:
- 7335 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Trachtmann N, Alvarez Fong KF, Guitart Font E, Sprenger GA.
pJLIC 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pJLIC 载体序列
LOCUS 40924_26231 7335 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pJLIC, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7335)
AUTHORS Trachtmann N, Alvarez Fong KF, Guitart Font E, Sprenger GA.
TITLE Construction of chromosomally encoded lacZ and gfp reporter strains
of Escherichia coli for global regulation of metabolism
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7335)
AUTHORS Trachtmann N, Alvarez Fong KF, Guitart Font E, Sprenger GA.
TITLE Direct Submission
JOURNAL Submitted (24-MAY-2016) Institute of microbiology, Stuttgart
university, Allmandring 31, Stuttgart 70569, Germany
REFERENCE 3 (bases 1 to 7335)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7335)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(24-MAY-2016) Institute of microbiology, Stuttgart university,
Allmandring 31, Stuttgart 70569, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7335
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 6..179
/label=lacZ-alpha
/note="LacZ-alpha fragment of beta-galactosidase"
regulatory 797..914
/label=5S terminator
/note="5S terminator"
/regulatory_class="terminator"
terminator 917..1003
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1095..1122
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1141..1232
/label=AmpR promoter
CDS 1233..2090
/label=AmpR
/note="beta-lactamase"
rep_origin 2264..2852
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(3038..3178)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3283..3471)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
CDS complement(4492..5571)
/label=lacI
/note="lac repressor"
promoter complement(5572..5649)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
misc_feature 5754..6080
/label=flanking arm for integration region
/note="flanking arm for integration region"
protein_bind complement(6111..6158)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS complement(6194..6832)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/function="chloramphenicol resistance"
/label=cat
/protein_id="APZ86789.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR"
gene complement(6194..6832)
/gene="cat"
/label=cat
promoter complement(6833..6935)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(7041..7074)
/label=FRT (minimal)
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter 7266..7294
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 7302..7318
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."