载体基本信息 | |||
载体名称 | pUGW14 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 6130 bp | 载体类型 | Gateway vector |
载体来源 | Tanaka Y, Nakagawa T. |
pUGW14 载体载体图谱
pUGW14 载体载体序列
LOCUS Exported 6130 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Gateway vector pUGW14 DNA, complete sequence. ACCESSION AB626671 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6130) AUTHORS Tanaka Y, Nakagawa T. TITLE Gateway Vectors for transient expression JOURNAL Unpublished REFERENCE 2 (bases 1 to 6130) AUTHORS Tanaka Y, Nakagawa T. TITLE Direct Submission JOURNAL Submitted (19-APR-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center for Integrated Research in Science; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan REFERENCE 3 (bases 1 to 6130) AUTHORS . TITLE Direct Submission COMMENT constructed using pUC119. FEATURES Location/Qualifiers source 1..6130 /organism="Gateway vector pUGW14" /mol_type="other DNA" /db_xref="taxon:1007965" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" regulatory 276..1109 /regulatory_class="promoter" /note="35S promoter" promoter 763..1108 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature complement(1130..1254) /label=attR1 /note="attR1" protein_bind 1130..1254 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1291..1321 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1375..2034 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=cat /protein_id="BAK19548.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" gene 1375..2034 /gene="cat" /label=cat CDS 2376..2681 /codon_start=1 /gene="ccdB" /label=ccdB /protein_id="BAK19549.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" gene 2376..2681 /gene="ccdB" /label=ccdB misc_feature 2722..2846 /label=attR2 /note="attR2" protein_bind complement(2722..2846) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 2857..2976 /label=3xHA /note="3xHA" CDS 2875..2964 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA" regulatory 2982..3258 /regulatory_class="terminator" /note="NOS terminator" terminator 2998..3250 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3259..3275) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3488..3943 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4225..4329 /gene="bla" /label=AmpR promoter CDS 4330..5190 /codon_start=1 /gene="bla" /product="beta lactamase" /label=bla /protein_id="BAK19550.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene 4330..5190 /gene="bla" /label=bla rep_origin complement(5351..5951) /direction=LEFT /label=ColE1 Ori /note="ColE1 Ori" rep_origin 5361..5949 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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