载体基本信息 | |||
载体名称 | pVMG-TnpR | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 7264 bp | 载体类型 | Cloning vector |
载体来源 | Oke V, Long SR. |
pVMG-TnpR 载体载体图谱
pVMG-TnpR 载体载体序列
LOCUS Exported 7264 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pVMG-TnpR, complete sequence. ACCESSION EU232661 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7264) AUTHORS Oke V, Long SR. TITLE Bacterial genes induced within the nodule during the Rhizobium-legume symbiosis JOURNAL Mol. Microbiol. 32 (4), 837-849 (1999) PUBMED 10361286 REFERENCE 2 (bases 1 to 7264) AUTHORS Gao M, Teplitski M. TITLE RIVET-a tool for in vivo analysis of symbiotically relevant gene expression in Sinorhizobium meliloti JOURNAL Mol. Plant Microbe Interact. 21 (2), 162-170 (2008) PUBMED 18184060 REFERENCE 3 (bases 1 to 7264) AUTHORS Gao M, Oke V, Teplitski M. TITLE Direct Submission JOURNAL Submitted (17-OCT-2007) Soil and Water Science, University of Florida-IFAS, 1376 Mowry Rd., Cancer & Genetics Research Center, Gainesville, FL 32611, USA REFERENCE 4 (bases 1 to 7264) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..7264 /organism="Cloning vector pVMG-TnpR" /mol_type="other DNA" /label=for constructing tnpR-gus promoter probe fusions /note="for constructing tnpR-gus promoter probe fusions" /db_xref="taxon:481817" regulatory 28..58 /regulatory_class="terminator" /note="Escherichia coli trpA" misc_feature 103..113 /label=contains stop codons in all three reading frames /note="contains stop codons in all three reading frames" gene 189..756 /gene="tnpR" /label=tnpR regulatory 189..195 /regulatory_class="ribosome_binding_site" /gene="tnpR" CDS 205..756 /codon_start=1 /gene="tnpR" /product="gamma-delta resolvase" /label=tnpR /note="TnpR; site-specific recombinase; from pGOA1193" /protein_id="ABX47149.1" /translation="MRLFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKASGSSCDR KGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDGEMG KMVVTILSAVAQAERQRILERTNEGRQEAMAKGVVFGRKRKIDRDAVLNMWQQGLGASH ISKTMNIARSTVYKVINESN" gene 756..2600 /gene="uidA" /label=uidA regulatory 756..761 /regulatory_class="ribosome_binding_site" /gene="uidA" CDS 788..2599 /codon_start=1 /gene="uidA" /product="beta-glucuronidase" /label=uidA /note="UidA; Gus; b-glucuronidase; from pVO155" /protein_id="ABX47150.1" /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK PKSAAFLLQKRWTGMNFGEKPQQGGKQ" gene 3019..3814 /gene="nptII" /label=nptII CDS 3019..3813 /codon_start=1 /gene="nptII" /product="neomycin phosphotransferase" /label=nptII /note="NptII; aminoglycoside-3'-O-phosphotransferase; neomycin/kanamycin resistance; from pVO155" /protein_id="ABX47151.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4183..4199) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4412..4867 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" oriT 5129..5450 oriT 5206..5315 /note="incP origin of transfer" promoter 5485..5589 /gene="bla" /label=AmpR promoter gene 5590..6451 /gene="bla" /label=bla CDS 5590..6450 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=bla /note="Bla; b-lactamase; ampicillin resistance" /protein_id="ABX47152.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 6621..7209 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 6635..7261 /label=ColE1 /note="ColE1"
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