| 载体基本信息 | |||
| 载体名称 | pC0056 - LwCas13a-msfGFP-NES | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 13585 bp | 载体类型 | Mammalian Expression, Lentiviral |
| 拷贝数 | High Copy | ||
pC0056 - LwCas13a-msfGFP-NES 载体载体图谱
pC0056 - LwCas13a-msfGFP-NES 载体载体序列
LOCUS pC0056_-_LwCas13 13585 bp DNA circular SYN 13-MAY-2021
DEFINITION Expresses active LwaCas13a-NES for mammalian RNA targeting.
ACCESSION .
VERSION .
KEYWORDS pC0056 - LwCas13a-msfGFP-NES.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13585)
AUTHORS Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J,
Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES,
Voytas DF, Ting AY, Zhang F
TITLE RNA targeting with CRISPR-Cas13.
JOURNAL Nature. 2017 Oct 4. doi: 10.1038/nature24049.
PUBMED 28976959
REFERENCE 2 (bases 1 to 13585)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 13585)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature.
2017 Oct 4. doi: 10.1038/nature24049."
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13585
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 618..820
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2291..2332
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2682..3860
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 3915..7370
/label=LwCas13a
/note="Leptotrichia wadei CRISPR-Cas effector that acts as
an RNA-guided RNAse (Abudayyeh et al., 2017)"
CDS 7416..8129
/label=superfolder GFP
/note="GFP variant that folds robustly even when fused to
poorly folded proteins (Pedelacq et al., 2006)"
CDS 8169..8195
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 8196..8222
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 8223..8249
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
misc_feature 8277..8865
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8868..8884)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8869..8885)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 9390..9570
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 9602..9826
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9872..10300
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9959..9978)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 10169..10190
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 10314..10643
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 10691..10738
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 10757..11128
/label=BleoR
/note="antibiotic-binding protein"
polyA_signal 11261..11394
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11431..11447)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11431..11447)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11444..11466)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11455..11471
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11479..11509)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(11524..11545)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11662..11679)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11833..12421)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12595..13452)
/label=AmpR
/note="beta-lactamase"
promoter complement(13453..13557)
/label=AmpR promoter
我们不提供该载体，载体信息仅供查询和参考。