载体基本信息 | |||
载体名称 | GmScream8-pFLEV | 载体抗性 | Ampicillin |
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载体长度 | 5206 bp | 载体类型 | Cloning vector |
载体来源 | Zhang N, McHale LK, Finer JJ. |
GmScream8-pFLEV 载体载体图谱
GmScream8-pFLEV 载体载体序列
LOCUS Exported 5206 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GmScream8-pFLEV, complete sequence. ACCESSION KX252724 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5206) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 5206) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 5206) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5206 /organism="Cloning vector GmScream8-pFLEV" /mol_type="other DNA" /label=derived from Glycine max /note="derived from Glycine max" /db_xref="taxon:1890317" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 405..1923 /regulatory_class="promoter" /note="GmScream8 promoter" CDS 1926..2645 /codon_start=1 /product="sGFP65T" /label=sGFP65T /protein_id="AOC59747.1" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" regulatory 2678..2953 /regulatory_class="terminator" /note="NOS terminator" terminator 2678..2930 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(2985..3001) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 3009..3025 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3033..3063) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3078..3099 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3387..3975) /direction=LEFT misc_feature complement(4146..5006) /label=derived from ampicillin gene /note="derived from ampicillin gene" CDS complement(4146..5006) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5007..5111) /gene="bla" /label=AmpR promoter
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