载体基本信息 | |||
载体名称 | pC1300intB-35SnosBK | 载体抗性 | Kanamycin |
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载体长度 | 9946 bp | 载体类型 | Cloning vector |
载体来源 | Ouwerkerk PBF., Meijer AH. |
pC1300intB-35SnosBK 载体载体图谱
pC1300intB-35SnosBK 载体载体序列
LOCUS Exported 9946 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pC1300intB-35SnosBK, complete sequence. ACCESSION AY560326 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9946) AUTHORS Ouwerkerk PBF., Meijer AH. TITLE Binary expression vector for use in plants JOURNAL Unpublished REFERENCE 2 (bases 1 to 9946) AUTHORS Ouwerkerk PBF., Meijer AH. TITLE Direct Submission JOURNAL Submitted (26-FEB-2004) Molecular Cell Biology, Institute of Biology, Leiden University, Wassenaarseweg 64, Leiden 2333 AL, The Netherlands REFERENCE 3 (bases 1 to 9946) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9946 /organism="Cloning vector pC1300intB-35SnosBK" /mol_type="other DNA" /db_xref="taxon:267431" terminator 48..300 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" regulatory complement(48..299) /regulatory_class="terminator" /note="3'NOS; terminator signal of nopaline synthase gene" misc_feature complement(318..356) /note="multiple cloning site; SalI, EcoRI, XhoI, BglII, KpnI, SpeI are unique" misc_feature 358..413 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" regulatory complement(434..798) /regulatory_class="promoter" /note="CaMV 35S promoter" promoter complement(435..481) /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" primer_bind complement(837..853) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 1056..1080 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" misc_feature 1075..1100 /label=right border T-DNA repeat /note="right border T-DNA repeat" misc_feature complement(2141..3141) /label=STA region from pVS1 plasmid /note="STA region from pVS1 plasmid" CDS 2380..3009 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 3443..4510 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin complement(3734..4734) /direction=LEFT /note="pVS1-REP; replication origin from pVS1" rep_origin 4576..4770 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 5114..5254 /label=bom /note="basis of mobility region from pBR322" misc_feature complement(5144..5404) /label=bom site from pBR322 /note="bom site from pBR322" rep_origin complement(5440..6028) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin complement(5544..5824) /direction=LEFT /label=pBR322 origin of replication /note="pBR322 origin of replication" CDS complement(6115..6909) /codon_start=1 /gene="aadA" /product="aminoglycoside transferase" /label=aadA /note="AadA; kanamycin resistance gene from pIG121Hm" /protein_id="AAS73285.1" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" gene complement(6115..6909) /gene="aadA" /label=aadA misc_feature complement(7334..7359) /label=left border repeat from C58 T-DNA /note="left border repeat from C58 T-DNA" misc_feature 7334..7358 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" regulatory complement(7426..7645) /regulatory_class="polyA_signal_sequence" /note="CaMV polyA signal" polyA_signal 7436..7610 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(join(7661..8347,8538..8876)) /codon_start=1 /gene="hptII" /product="hygromycin phosphotransferase" /label=hptII /note="HptII" /protein_id="AAS73286.1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" gene complement(7661..8876) /gene="hptII" /label=hptII exon complement(7661..8347) /gene="hptII" /number=1 intron complement(8348..8537) /gene="hptII" /note="derived from Ricinus communis catalase-1 intron presented in Genbank Accession Number D21161; confers higher resistance towards hygromycin in transgenic rice and allows easier killing of Agrobacterium tumefaciens during regeneration of transgenic plants" exon complement(8538..8876) /gene="hptII" /number=2 regulatory complement(8923..9703) /regulatory_class="promoter" /note="35S promoter from CaMV" promoter complement(8955..9632) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" protein_bind 9823..9844 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9859..9889 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9897..9913 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9921..9937 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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