| 载体基本信息 | |||
| 载体名称 | pBAC-TS | 载体抗性 | Tetracycline |
|---|---|---|---|
| 载体长度 | 10475 bp | 载体类型 | Cloning vector |
| 载体来源 | Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA, Del Vecchio D, Collins JJ. | ||
pBAC-TS 载体载体图谱
pBAC-TS 载体载体序列
LOCUS Exported 10475 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pBAC-TS, complete sequence.
ACCESSION KX264178
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10475)
AUTHORS Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
Del Vecchio D, Collins JJ.
TITLE Creating Single-Copy Genetic Circuits
JOURNAL Mol. Cell 63 (2), 329-336 (2016)
PUBMED 27425413
REFERENCE 2 (bases 1 to 10475)
AUTHORS Lee JW, Gyorgy A, Cameron DE, Pyenson N, Choi KR, Way JC, Silver PA,
Del Vecchio D, Collins JJ.
TITLE Direct Submission
JOURNAL Submitted (18-MAY-2016) Biological Engineering, MIT, 45 Carleton St,
MIT Building E25-Rm302, Cambridge, MA 02142, USA
REFERENCE 3 (bases 1 to 10475)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..10475
/organism="Cloning vector pBAC-TS"
/mol_type="other DNA"
/db_xref="taxon:1883154"
CDS complement(102..1073)
/codon_start=1
/gene="sopB"
/product="partitioning protein for the bacterial F plasmid"
/label=sopB
/translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
CDS complement(1073..2248)
/codon_start=1
/gene="sopA"
/product="partitioning protein for the bacterial F plasmid"
/label=sopA
/translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
misc_feature 2574..2824
/gene="incC"
/label=incC
/note="incompatibility region of the bacterial F plasmid"
CDS complement(2827..3582)
/codon_start=1
/gene="repE"
/product="replication initiation protein for the bacterial
F plasmid"
/label=repE
/translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
rep_origin 3673..3892
/label=ori2
/note="secondary origin of replication for the bacterial F
plasmid; also known as oriS"
rep_origin 3674..3891
/label=OriS origin
/note="OriS origin"
rep_origin 3962..4586
/label=OriV origin
/note="OriV origin"
rep_origin 3968..4582
/label=oriV
/note="origin of replication for the bacterial F plasmid"
promoter 5440..5542
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 5543..6202
/codon_start=1
/product="CamR"
/label=CamR
/protein_id="ANW61907.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
regulatory 6331..6436
/regulatory_class="terminator"
/note="T0 transcriptional terminator from phage lambda"
terminator 6337..6431
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(6491..7207)
/codon_start=1
/product="green fluorescent protein mut3b"
/label=green fluorescent protein mut3b
/note="gfp(mut3b)"
/protein_id="ANW61911.1"
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
regulatory complement(7259..7332)
/regulatory_class="promoter"
/note="PLtetO"
promoter complement(7259..7332)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
prim_transcript 7278
/label=transcription start
/note="transcription start"
protein_bind 7289..7307
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 7314..7332
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS complement(7375..8538)
/codon_start=1
/product="LacI"
/label=LacI
/protein_id="ANW61910.1"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQAANKNEENTNEVPTFMLNAGQANYLSQ"
misc_feature complement(7375..7458)
/label=mfLon-specific tag pdt2
/note="mfLon-specific tag pdt2"
regulatory complement(8575..8648)
/regulatory_class="promoter"
/note="PLtetO"
promoter complement(8575..8648)
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
prim_transcript 8594
/label=transcription start
/note="transcription start"
protein_bind 8605..8623
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8630..8648
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 8667..8741
/regulatory_class="promoter"
/note="Ptrc-2"
promoter 8680..8709
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
prim_transcript 8715
/label=transcription start
/note="transcription start"
protein_bind 8717..8733
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 8780..9403
/codon_start=1
/product="TetR"
/label=TetR
/protein_id="ANW61909.1"
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
regulatory 9446..9520
/regulatory_class="promoter"
/note="Ptrc-2"
promoter 9459..9488
/label=trc promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 9496..9512
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
prim_transcript 9496
/label=transcription start
/note="transcription start"
CDS 9563..10273
/codon_start=1
/product="mCherry"
/label=mCherry
/protein_id="ANW61908.1"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
regulatory 10328..10432
/regulatory_class="terminator"
/note="T1 transcriptional terminator from rrnB operon"
terminator 10333..10419
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
我们不提供该载体，载体信息仅供查询和参考。