pGreenII 62-SK is a plant expression vector.
| 载体基本信息 | |||
| 载体名称 | pGreenII 62-SK | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 3347 bp | 载体类型 | Plant Expression |
| 启动子 | CaMV 35S | ||
pGreenII 62-SK 载体载体图谱
pGreenII 62-SK 载体载体序列
LOCUS Exported 3347 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3347)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3347
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 542..564
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 638..982
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 992..1099
/note="MCS"
/note="pBluescript multiple cloning site"
primer_bind 1016..1032
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1066..1082)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 1153..1329
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 1339..1363
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(1454..2042)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2213..3028)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 3319..407
/note="pSa ori"
/note="origin of replication from bacterial plasmid pSa"
我们不提供该载体，载体信息仅供查询和参考。