载体基本信息 | |||
载体名称 | pCMV-TALER31 | 载体抗性 | Kanamycin |
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载体长度 | 7380 bp | 载体类型 | Cloning vector |
载体来源 | Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z. |
pCMV-TALER31 载体载体图谱
pCMV-TALER31 载体载体序列
LOCUS Exported 7380 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pCMV-TALER31, complete sequence. ACCESSION KM486841 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7380) AUTHORS Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z. TITLE Modular construction of mammalian gene circuits using TALE transcriptional repressors JOURNAL Unpublished REFERENCE 2 (bases 1 to 7380) AUTHORS Li Y, Jiang Y, Chen H, Liao W. TITLE Direct Submission JOURNAL Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic & Systems Biology, Tsinghua National Laboratory for Information Science and Technology, Tsinghua University, Beijing 100084, China REFERENCE 3 (bases 1 to 7380) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7380 /organism="Cloning vector pCMV-TALER31" /mol_type="other DNA" /db_xref="taxon:1579874" polyA_signal 190..311 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 318..773 /label=f1 origin /note="f1 origin" promoter 800..904 /gene="bla" /label=AmpR promoter promoter 906..1263 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1114..1249 /label=SV40 ori /note="SV40 origin of replication" CDS 1298..2092 /codon_start=1 /product="KanR/NeoR" /label=KanR/NeoR /protein_id="AIZ65986.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 2324..2371 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 2674..3347 /label=pUC origin /note="pUC origin" rep_origin 2700..3288 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature 3403..3991 /label=CMV /note="CMV" enhancer 3463..3766 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3767..3970 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 4005..4721 /label=similar to EYFP /note="similar to EYFP" CDS 4005..4721 /codon_start=1 /product="enhanced YFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" misc_feature 4728..4781 /label=2A /note="2A" CDS 4728..4781 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" misc_feature 4788..7325 /label=similar to TALER31 /note="similar to TALER31" CDS 7332..7352 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV"
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