pQLinkG 载体 (V003853#)

载体名称:
pQLinkG
载体抗性:
Ampicillin
载体长度:
5537 bp
载体类型:
Cloning vector
复制子:
ori
载体来源:
Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.

pQLinkG 载体载体图谱

pQLinkG5537 bp60012001800240030003600420048005400LIC cassette with PacI restriction siteT5 promoterlac operatorRBSGSTattB1TEV siteattB2lambda t0 terminatorLIC cassette with SwaI, PacI restriction sitesCmRrrnB T1 terminatorCAP binding sitelacIlacIq promoterbomoriAmpRAmpR promoter

pQLinkG 载体载体序列

LOCUS       40924_36208        5537 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQLinkG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
  TITLE     Vectors for co-expression of an unrestricted number of proteins
  JOURNAL   Nucleic Acids Res. 35 (6), E43 (2007)
  PUBMED    17311810
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Buessow K, Scheich C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2006) Vertebrate Genomics, Max Planck Institute 
            for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 5537)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5537)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35"; issue: "6"; pages: "E43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2006) Vertebrate Genomics, Max Planck Institute for 
            Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..27
                     /label=LIC cassette with PacI restriction site
                     /note="LIC cassette with PacI restriction site"
     promoter        36..80
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      127..133
                     /regulatory_class="ribosome_binding_site"
     CDS             141..794
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     protein_bind    817..837
                     /label=attB1
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             840..860
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     protein_bind    complement(898..922)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      944..1038
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    1043..1092
                     /label=LIC cassette with SwaI, PacI restriction sites
                     /note="LIC cassette with SwaI, PacI restriction sites"
     CDS             1132..1788
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1856..1942
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(2001..2022)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2038..3117)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3118..3195)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3387..3527
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      3711
                     /label=ColE1 ori
                     /note="ColE1 ori"
     rep_origin      complement(3713..4301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4475..5332)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5333..5437)
                     /label=AmpR promoter

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