载体基本信息 | |||
载体名称 | pQLinkG | 载体抗性 | Ampicillin |
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载体长度 | 5537 bp | 载体类型 | Cloning vector |
载体来源 | Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K. |
pQLinkG 载体载体图谱
pQLinkG 载体载体序列
LOCUS Exported 5537 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pQLinkG, complete sequence. ACCESSION EF025689 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5537) AUTHORS Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K. TITLE Vectors for co-expression of an unrestricted number of proteins JOURNAL Nucleic Acids Res. 35 (6), E43 (2007) PUBMED 17311810 REFERENCE 2 (bases 1 to 5537) AUTHORS Buessow K, Scheich C. TITLE Direct Submission JOURNAL Submitted (25-SEP-2006) Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany REFERENCE 3 (bases 1 to 5537) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..5537 /organism="Cloning vector pQLinkG" /mol_type="other DNA" /db_xref="taxon:405934" misc_feature 7..27 /label=LIC cassette with PacI restriction site /note="LIC cassette with PacI restriction site" promoter 36..80 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 56..72 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." regulatory 56..61 /regulatory_class="minus_35_signal" regulatory 74..79 /regulatory_class="minus_10_signal" protein_bind 88..104 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 123..134 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" regulatory 127..133 /regulatory_class="ribosome_binding_site" CDS 141..896 /codon_start=1 /gene="gst" /product="glutathione S-transferase" /label=gst /protein_id="ABJ15774.1" /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLG AAGTSLYKKAGENLYFQGSSLRMHEDAAA" gene 141..896 /gene="gst" /label=gst CDS 141..794 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK" protein_bind 816..840 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 840..860 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" misc_feature 858..928 /note="multiple cloning site; includes BamHI, NotI and HindIII restriction sites" protein_bind complement(898..922) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 944..1038 /regulatory_class="terminator" /note="lambda t0 transcriptional terminator" terminator 944..1038 /label=lambda t0 terminator /note="transcription terminator from phage lambda" misc_feature 1043..1092 /label=LIC cassette with SwaI, PacI restriction sites /note="LIC cassette with SwaI, PacI restriction sites" CDS 1132..1791 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" regulatory 1850..1947 /regulatory_class="terminator" /note="rrnB T1 transcriptional terminator" terminator 1856..1942 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(2035..3117) /codon_start=1 /gene="lacI" /allele="Q" /product="lac repressor" /label=lacI /note="overproducing allele; similar to lac repressor encoded by GenBank Accession Number U78872" /protein_id="ABJ15773.1" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" gene complement(2035..3117) /gene="lacI" /allele="Q" /label=lacI promoter complement(3118..3195) /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." misc_feature 3387..3527 /label=bom /note="basis of mobility region from pBR322" rep_origin 3711 /label=ColE1 ori /note="ColE1 ori" rep_origin complement(3713..4301) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4472..5332) /codon_start=1 /gene="bla" /product="beta lactamase" /label=bla /note="penicillinase; similar to the protein encoded by GenBank Accession Number AF074376" /protein_id="ABJ15772.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(4472..5332) /gene="bla" /label=bla promoter complement(5333..5437) /gene="bla" /label=AmpR promoter
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