载体基本信息 | |||
载体名称 | pP02218-nat-cel8A | 载体抗性 | Ampicillin |
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载体长度 | 6904 bp | 载体类型 | Shuttle vector |
载体来源 | Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov VV, Liebl W, Kornberger P. |
pP02218-nat-cel8A 载体载体图谱
pP02218-nat-cel8A 载体载体序列
LOCUS Exported 6904 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector pP02218-nat-cel8A, complete sequence. ACCESSION MH328020 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6904) AUTHORS Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov VV, Liebl W, Kornberger P. TITLE Efficient secretory heterologous enzyme production in the novel host Paenibacillus polymyxa by usage of its promoter sequences JOURNAL Unpublished REFERENCE 2 (bases 1 to 6904) AUTHORS Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov VV, Liebl W, Kornberger P. TITLE Direct Submission JOURNAL Submitted (09-MAY-2018) Department for Microbiology, Technical University of Munich, Emil-Ramann-Str. 4, Freising, Bavaria 85354, Germany REFERENCE 3 (bases 1 to 6904) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6904 /organism="Shuttle vector pP02218-nat-cel8A" /mol_type="other DNA" /label=derived from Escherichia coli TOP10 /note="derived from Escherichia coli TOP10" /db_xref="taxon:2282830" regulatory complement(77..676) /regulatory_class="replication_regulatory_region" /note="ColE1 origin of replication, E. coli" rep_origin complement(79..667) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(838..1698) /codon_start=1 /gene="ampR" /product="AmpR" /label=ampR /note="Ampicillin Resistance" /protein_id="AXF54529.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" gene complement(838..1698) /gene="ampR" /label=ampR promoter complement(1699..1803) /gene="bla" /label=AmpR promoter regulatory complement(1832..2353) /regulatory_class="other" /note="origin of transfer for conjugation, contains traJ and oriT" CDS complement(1832..2116) /codon_start=1 /gene="traJ" /product="TraJ" /label=traJ /protein_id="AXF54530.1" /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV GQGYKITGVVDYEHVRELARINGDLGQQKAHYVNHHPNQVFWGRGAVKH" gene complement(1832..2116) /gene="traJ" /label=traJ regulatory complement(2117..2353) /regulatory_class="other" /note="oriT (origin of transfer)" oriT 2149..2258 /note="incP origin of transfer" CDS complement(2515..3276) /codon_start=1 /gene="kanR" /product="KanR" /label=kanR /note="Kanamycin Resistance" /protein_id="AXF54531.1" /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK LLESLENFWNGIQEWTERHGYIVDVSKRIPF" gene complement(2515..3276) /gene="kanR" /label=kanR regulatory complement(3445..4612) /regulatory_class="replication_regulatory_region" /note="pUB origin of replication, Bacillus" CDS complement(3445..4449) /codon_start=1 /gene="repB" /product="RepB replication protein" /label=repB /note="from Enterococcus faecalis plasmid pAM-alpha-1" /db_xref="GI:22652809" /protein_id="AAN03827.1" /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE" regulatory 4866..5277 /regulatory_class="promoter" /note="P02218 promoter from P. polymyxa DSM292" gene 5275..6744 /gene="SPnat-Cel8A-His6" /label=SPnat-Cel8A-His6 CDS 5275..5365 /codon_start=1 /gene="SPnat-Cel8A-His6" /product="signal peptide of P. polymyxa DSM292 ORF PPOLYM_02218" /label=SPnat-Cel8A-His6 /protein_id="AXF54532.1" /translation="MEKSKLLGLTALSALTLSLSLIPTQQTYAE" CDS 5383..6744 /codon_start=1 /gene="SPnat-Cel8A-His6" /product="Cel8A with C-terminal hexahistidine-tag" /label=SPnat-Cel8A-His6 /protein_id="AXF54533.1" /translation="AGVPFNTKYPYGPTSIADNQSEVTAMLKAEWEDWKSKRITSNGAG GYKRVQRDASTNYDTVSEGMGYGLLLAVCFNEQALFDDLYRYVKSHFNGNGLMHWHIDA NNNVTSHDGGDGAATDADEDIALALIFADKLWGSSGAINYGQEARTLINNLYNHCVEHG SYVLKPGDRWGGSSVTNPSYFAPAWYKVYAQYTGDTRWNQVADKCYQIVEEVKKYNNGT GLVPDWCTASGTPASGQSYDYKYDATRYGWRTAVDYSWFGDQRAKANCDMLTKFFARDG AKGIVDGYTIQGSKISNNHNASFIGPVAAASMTGYDLNFAKELYRETVAVKDSEYYGYY GNSLRLLTLLYITGNFPNPLSDLSGQPTPPSNPTPSLPPQVVYGDVNGDGNVNSTDLTM LKRYLLKSVTNINREAADVNRDGAINSSDMTILKRYLIKSIPHLPYSRHHHHHH" CDS 6724..6741 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH"
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