载体基本信息 | |||
载体名称 | pST-2K | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 4961 bp | 载体类型 | Dual expression shuttle vector |
载体来源 | Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, Nandicoori VK. |
pST-2K 载体载体图谱
pST-2K 载体载体序列
LOCUS Exported 4961 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Dual expression shuttle vector pST-2K, complete sequence. ACCESSION KC153032 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4961) AUTHORS Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, Nandicoori VK. TITLE Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria JOURNAL Appl. Environ. Microbiol. 79 (5), 1718-1729 (2013) PUBMED 23315736 REFERENCE 2 (bases 1 to 4961) AUTHORS Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, Nandicoori VK. TITLE Direct Submission JOURNAL Submitted (11-NOV-2012) Signal Transduction Lab-I, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, Delhi 110067, India REFERENCE 3 (bases 1 to 4961) AUTHORS . TITLE Direct Submission COMMENT ##Assembly-Data-START## Assembly Method :: ClustalW v. 2.1 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4961 /organism="Dual expression shuttle vector pST-2K" /mol_type="other DNA" /db_xref="taxon:1283727" misc_feature 7..308 /note="Pmyc1tetO; UV15 promoter with tetO" protein_bind 179..196 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 315..321 /label=SD sequence 1 /note="SD sequence 1" misc_feature 332..352 /label=His tag /note="His tag" CDS 335..352 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 340..381 /label=MCS1 /note="MCS1" terminator 382..425 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory 383..443 /regulatory_class="terminator" /note="transcription terminator 1" regulatory 455..781 /regulatory_class="promoter" /note="UV15 promoter without tetO" misc_feature 784..790 /label=SD sequence 2 /note="SD sequence 2" misc_feature 791..820 /label=MCS2 /note="MCS2" misc_feature 821..847 /label=FLAG tag /note="FLAG tag" CDS 821..844 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" terminator 863..905 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" regulatory 881..998 /regulatory_class="terminator" /note="transcription terminator 2" terminator 934..980 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS 1141..1956 /codon_start=1 /product="kanamycin resistance protein" /label=kanamycin resistance protein /protein_id="AGE45659.1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 2259..3030 /label=oriE /note="oriE" rep_origin 2288..2876 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 3043..4936 /label=oriM /note="oriM"
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