载体基本信息 | |||
载体名称 | pTHSSe_53 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 3176 bp | 载体类型 | Cloning vector |
载体来源 | Segall-Shapiro TH, Sontag ED, Voigt CA. |
pTHSSe_53 载体载体图谱
pTHSSe_53 载体载体序列
LOCUS Exported 3176 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pTHSSe_53, complete sequence. ACCESSION MH029770 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3176) AUTHORS Segall-Shapiro TH, Sontag ED, Voigt CA. TITLE Engineered promoters enable constant gene expression at any copy number in bacteria JOURNAL Nat. Biotechnol. (2018) In press PUBMED 29553576 REFERENCE 2 (bases 1 to 3176) AUTHORS Segall-Shapiro TH, Sontag ED, Voigt CA. TITLE Direct Submission JOURNAL Submitted (06-MAR-2018) Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA REFERENCE 3 (bases 1 to 3176) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3176 /organism="Cloning vector pTHSSe_53" /mol_type="other DNA" /label=pUC BbsI cloning vector /note="pUC BbsI cloning vector" /db_xref="taxon:2137934" regulatory 13..60 /regulatory_class="promoter" /note="PJ23105; derived from BBa_J23105" ncRNA 61..135 /product="RiboJ" /label=ribozyme /note="insulator" /ncRNA_class="ribozyme" misc_RNA 63..112 /label=sTRSV HHRz /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)" regulatory 136..154 /regulatory_class="ribosome_binding_site" /note="B0032; derived from BBa_B0032" CDS 155..871 /codon_start=1 /product="sfGFP" /label=sfGFP /protein_id="AVR55154.1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" CDS 155..868 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" regulatory 880..1008 /regulatory_class="terminator" /note="B0015; BBa_B0015" terminator 888..959 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 975..1002 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 1021..1041 /label=BioBrick suffix /note="universal suffix for all parts" terminator 1042..1099 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." rep_origin 1195..1958 /label=pUC origin /note="pUC origin" rep_origin 1271..1859 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2054..2914) /codon_start=1 /product="TEM beta-lactamase" /label=TEM beta-lactamase /note="ampicillin resistance protein" /protein_id="AVR55153.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2915..3019) /gene="bla" /label=AmpR promoter terminator 3109..3152 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 3155..3176 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG"""
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