重组大肠杆菌BIRA蛋白

大肠杆菌

大肠杆菌

A DNA sequence encoding the E.coil BirA (P06709) (Met1–Lys321) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.

> 95 % as determined by SDS-PAGE

The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions.

B3973; DHBB; JW3941; BIOR

Bifunctional ligase/repressor BirA

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.

Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.