• KIPKASSVPTELSAISTLYL polypeptide is a highly promising bioactive peptide with significant potential in the field of bone tissue engineering. Derived from the knuckle epitope of bone morphogenetic protein 2 (BMP - 2), it exhibits a remarkable ability to induce osteogenic differentiation. This peptide has been shown to enhance the expression of osteogenic markers such as osteocalcin (OCN) and collagen I, making it a potent activator of the osteogenic signaling pathway.
• It offers several advantages over native proteins, including improved stability, cost - effectiveness, and ease of modification and immobilization onto scaffolds. With its dose - dependent effect on osteogenic gene expression, it provides a tunable approach for promoting bone formation. Overall, KIPKASSVPTELSAISTLYL polypeptide holds great promise as a therapeutic agent for bone regeneration and repair, and is a valuable tool for advancing research and applications in tissue engineering and regenerative medicine.

• Lukasova V, Buzgo M, Sovkova V, Dankova J, Rampichova M, Amler E. Osteogenic differentiation of 3D cultured mesenchymal stem cells induced by bioactive peptides. Cell Prolif. 2017 Aug;50(4):e12357. doi: 10.1111/cpr.12357. PMID: 28714176; PMCID: PMC6529093.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。